R&D Systems’ Luminex assays have the largest, most flexible Luminex analyte available. One LED identifies the analyte that is being detected and, the second LED determines the magnitude of the PE-derived signal. A magnet in the MAGPIX analyzer captures and holds the magnetic beads in a monolayer, while two spectrally distinct light-emitting diodes (LEDs) illuminate the beads. In addition to flow-based analyzers, magnetic beads can be read using the Luminex MAGPIX ® Analyzer. The second laser determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. One laser classifies the bead and determines the analyte that is being detected. It binds to the biotinylated detection antibodies.īeads are read on a dual-laser flow-based detection instrument, such as the Luminex 200 ™ or FlexMap ® analyzer. Phycoerythrin (PE)-conjugated streptavidin is added. The antibodies bind to the analytes of interest.īiotinylated detection antibodies specific to the analytes of interest are added and form an antibody-antigen sandwich. The sample is added to a mixture of color-coded beads, pre-coated with analyte-specific capture antibodies.
